Pressure myography, which is the standard for assessing vascular mechanics and vasoreactivity, is expen-
sive, has low throughput, and is limited to static fluid flow. Here, we developed HemoLens, an open-source
3D-printed pressure-myography system for <$750. HemoLens features compact micromanipulators, incre-
mental in-line pressure control, physiological temperature regulation, and modular pulse pressure control
between normotensive and hypertensive levels. HemoLens’s efficacy was demonstrated by delineation of
physiological reactivity and pathological mechanical phenotypes using native mouse arteries and bioprinted
acellular scaffolds. Engineered hypertensive vessels demonstrate increased burst pressure (464 mmHg) and
reduced dynamic compliance reminiscent of diseased arteries. Together, HemoLens lowers the barrier to en-
try in pressure-myography research by serving as a comprehensive low-cost system for native and engi-
neered vessel characterization.

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