Development of a Collagen-based 3D Bioprinted Microfluidic Platform for Vascular Tissue Engineering and Disease Modeling

• This proposal will use FRESH 3D bioprinting and a newly developed biomechanical strain sensor to 3D bioprint a functional resistance artery and create an ECM- based vascular microfluidic platform to improve our understanding of hypertensive disease progression.

Development of a Collagen-based 3D Bioprinted Microfluidic Platform for Vascular Tissue Engineering and Disease Modeling

• This proposal will use FRESH 3D bioprinting and a newly developed biomechanical strain sensor to 3D bioprint a functional resistance artery and create an ECM- based vascular microfluidic platform to improve our understanding of hypertensive disease progression.

Development of a Full-biologic 3D bioprinted Hepatic Sinusoid Model for Improved Risk Stratification of Thrombosis Burden in Fontan-treated Single-ventricle Patients

• This proposal aims to characterize the coagulation activity of Fontan recipients using a novel 3D bioprinted collagen-based microfluidics system. Initial results will focus on validating the model design, fabrication, and confirming the capability for a fully 3D bioprinted CHIPS to detect thrombosis via platelet aggregation and activation. 

Recreating Fontan-associated portal vein hypertension and fibrosis in a 3D bioprinted hepatic sinusoidal vascular system

• There is a critical need to develop an engineered model of Fontan Associated Liver Disease (FALD) to investigate the impact of single ventricle physiology on organ systems such as the liver. Our novel bioengineered solution will replicate FALD in the context of Fontan physiology to produce a human-specific model of liver fibrosis, assess blood clotting risk, identify predictive biomarkers, and enable future screening of patient-specific mutations correlating with FALD progression.